全文获取类型
收费全文 | 3964篇 |
免费 | 402篇 |
国内免费 | 6篇 |
出版年
2023年 | 29篇 |
2022年 | 16篇 |
2021年 | 116篇 |
2020年 | 63篇 |
2019年 | 69篇 |
2018年 | 81篇 |
2017年 | 77篇 |
2016年 | 143篇 |
2015年 | 191篇 |
2014年 | 204篇 |
2013年 | 215篇 |
2012年 | 291篇 |
2011年 | 283篇 |
2010年 | 200篇 |
2009年 | 160篇 |
2008年 | 262篇 |
2007年 | 237篇 |
2006年 | 247篇 |
2005年 | 217篇 |
2004年 | 178篇 |
2003年 | 182篇 |
2002年 | 153篇 |
2001年 | 46篇 |
2000年 | 27篇 |
1999年 | 38篇 |
1998年 | 34篇 |
1997年 | 21篇 |
1996年 | 27篇 |
1995年 | 34篇 |
1994年 | 22篇 |
1993年 | 19篇 |
1992年 | 23篇 |
1991年 | 25篇 |
1990年 | 17篇 |
1989年 | 16篇 |
1988年 | 20篇 |
1987年 | 11篇 |
1986年 | 20篇 |
1985年 | 17篇 |
1984年 | 24篇 |
1982年 | 20篇 |
1981年 | 14篇 |
1980年 | 25篇 |
1978年 | 17篇 |
1977年 | 19篇 |
1976年 | 13篇 |
1974年 | 18篇 |
1973年 | 13篇 |
1971年 | 13篇 |
1970年 | 11篇 |
排序方式: 共有4372条查询结果,搜索用时 203 毫秒
11.
Stephen M. Techtmann Julian L. Fortney Kati A. Ayers Dominique C. Joyner Thomas D. Linley Susan M. Pfiffner Terry C. Hazen 《PloS one》2015,10(3)
The waters of the Eastern Mediterranean are characterized by unique physical and chemical properties within separate water masses occupying different depths. Distinct water masses are present throughout the oceans, which drive thermohaline circulation. These water masses may contain specific microbial assemblages. The goal of this study was to examine the effect of physical and geological phenomena on the microbial community of the Eastern Mediterranean water column. Chemical measurements were combined with phospholipid fatty acid (PLFA) analysis and high-throughput 16S rRNA sequencing to characterize the microbial community in the water column at five sites. We demonstrate that the chemistry and microbial community of the water column were stratified into three distinct water masses. The salinity and nutrient concentrations vary between these water masses. Nutrient concentrations increased with depth, and salinity was highest in the intermediate water mass. Our PLFA analysis indicated different lipid classes were abundant in each water mass, suggesting that distinct groups of microbes inhabit these water masses. 16S rRNA gene sequencing confirmed the presence of distinct microbial communities in each water mass. Taxa involved in autotrophic nitrogen cycling were enriched in the intermediate water mass suggesting that microbes in this water mass may be important to the nitrogen cycle of the Eastern Mediterranean. The Eastern Mediterranean also contains numerous active hydrocarbon seeps. We sampled above the North Alex Mud Volcano, in order to test the effect of these geological features on the microbial community in the adjacent water column. The community in the waters overlaying the mud volcano was distinct from other communities collected at similar depths and was enriched in known hydrocarbon degrading taxa. Our results demonstrate that physical phenomena such stratification as well as geological phenomena such as mud volcanoes strongly affect microbial community structure in the Eastern Mediterranean water column. 相似文献
12.
13.
Intraspecific variation in reproductive physiology and egg quality in the European Starling Sturnus vulgaris 总被引:1,自引:0,他引:1
Egg mass shows large intraspecific variation in birds and is repeatable within individuals. The mechanisms underlying this variation are unknown. We hypothesized that measures of egg quality (the mass of yolk protein, yolk lipid, and albumen protein) would be positively correlated with the plasma pools of the yolk precursor vitellogenin, and the masses of the oviduct, metabolic machinery (liver, heart, lungs, kidneys, gizzard, small intestine and pancreas), and endogenous stores of protein and lipid. We tested these predictions in European Starlings Sturnus vulgaris collected at the peak of egg production effort. In contrast to our predictions, both yolk protein and yolk lipid were negatively correlated with plasma vitellogenin levels. Albumen protein was positively related to oviduct mass, but other aspects of body composition failed to explain variation in egg quality. Hence, while we observed correlations between egg composition and peripheral systems (circulating precursor pools and the oviduct), we found no evidence that egg quality is determined by more general processes, i.e., the supply and processing of nutrients. 相似文献
14.
Expression of desmin, laminin and fibronectin during in situ differentiation (decidualization) of rat uterine stromal cells 总被引:2,自引:0,他引:2
Stanley R. Glasser Saara Lampelo M. Idrees Munir JoAnne Julian 《Differentiation; research in biological diversity》1987,35(2):132-142
Immunocytochemical analysis of frozen rat uterine sections containing decidual tissue, formed in response to normal or artificial stimulation of uteri sensitized by endogenous or exogenous hormonal regimens, demonstrated an elevated expression of the intermediate filament protein desmin in decidual cells. Changes in the expression of extracellular matrix (ECM) components were coordinated with the elevated expression of desmin as stromal cells underwent decidualization. In parallel with the pattern of regional decidualization, as determined by elevated desmin expression, laminin accumulated in ECM of decidual cells while an apparent decrease in fibronectin was associated with altered organization at the decidual cell surface. The in situ observations confirm previous results, which indicated that the expression of desmin in decidual cells formed in vivo or in vitro is a valid marker of their differentiation, and resolve questions unanswered in the previous study: (a) desmin (and laminin) appear to be constitutively expressed in non-decidualized stroma at barely detectable levels, (b) desmin is a valid marker of stromal cell differentiation because it is expressed similarly in decidual cells, irrespective of varying experimental protocols for uterine sensitization and stimulation, and (c) desmin expression follows the same regional progression described for the process of decidualization in morphological and histochemical studies. 相似文献
15.
Aminoglycoside suppression at UAG, UAA and UGA codons in Escherichia coli and human tissue culture cells 总被引:6,自引:0,他引:6
Robin Martin Anne E. Mogg Louise A. Heywood Lars Nitschke Julian F. Burke 《Molecular & general genetics : MGG》1989,217(2-3):411-418
Summary We have compared the suppression of nonsense mutations by aminoglycoside antibiotics inEscherichia coli and in human 293 cells. Six nonsense alleles of the chloramphenicol acetyl transferase (cat) gene, in the vector pRSVcat, were suppressed by growth in G418 and paromomycin. Readthrough at UAG, UAA and UGA codons was monitored with enzyme assays
for chloramphenicol acetyl transferase (CAT), in stably transformed bacteria and during transient expression from the same
plasmid in human 293 tissue culture cells. We have found significant differences in the degree of suppression amongst three
UAG codons and two UAA codons in different mRNA contexts. However, the pattern of these effects are not the same in the two
organisms. Our data suggest that context effects of nonsense suppression may operate under different rules inE. coli and human cells. 相似文献
16.
C Julian M C Tordo G Macquart-Moulin J P Moatti F Giraud S Aymé 《Social biology》1989,36(3-4):240-247
A study of factors influencing genetic counseling attendance rate has been conducted in the Bouches-du-Rh?ne area, in the south of France. In this area, a birth defects monitoring system (Eurocat n. 22) annually covers 23,000 births. All the genetic services are delivered by only one genetic center located in Marseilles, and the data collected are computerized. The comparison of these two data bases gives an opportunity to estimate the rate of genetic counseling attendance after the occurrence of an affected stillbirth or live birth. Among the parents of 358 infants born in 1983-84 in this area with a pathology requiring genetic counseling, 226 (63 per cent) attended the Genetic Center within the first year after birth. The rate of attendance is statistically higher (p less than 0.01) for the parents who had a stillbirth (78 per cent) than for those who had a live birth (57 per cent). It is also higher (p less than 0.01) for the Marseilles maternities group (68 per cent) than for the group outside Marseilles (50 per cent). The referral delays are also analyzed according to malformation etiology and to viability of the child by the eighth day of life. Besides evaluating a particular genetic center's effectiveness in diffusing information to the public concerned, this work shows that couples' request is strongly dependent on a psychological need. 相似文献
17.
Summary The accumulation of interferon (IFN) -2 in transformed strains of Escherichia coli and Methylophilus methylotrophus was greater at 25° C than at 37° C. Interferon -2 catabolism was followed by measuring the change in IFN titre (measured immunoreactively) with time at temperatures between 25° C and 37° C in chloramphenicol-treated cells. The IFN -2 titre remained constant at 29° C and below, while at higher temperatures the titres declined. The t
1/2 values for IFN -2 decreased with increasing incubation temperature. Pulse-chase studies using [35S]methionine, sodium dodecyl sulphate-gel electrophoresis and autoradiography demonstrated that IFN -2 was subjected to degradation at 37° C while at 25° C it was stable. It is proposed that the susceptibility of IFN -2 to degradation in both E. coli and M. methylotrophus is affected by incubation temperature and 30° C may be a transition temperature above which the conformation of the molecule is recognised by the bacterial proteases. 相似文献
18.
Effects of DNase production, plasmid size, and restriction barriers on transformation of Vibrio cholerae by electroporation and osmotic shock 总被引:14,自引:0,他引:14
Hilda Marcus Julian M. Ketley James B. Kaper Randall K. Holmes 《FEMS microbiology letters》1990,68(1-2):149-154
Attempts to transform wild type strains of V. cholerae with plasmid DNA by traditional osmotic shock methods were not successful. A mutant of V. cholerae that was deficient in extracellular DNase was transformed with plasmid DNA by osmotic shock, demonstrating directly that extracellular DNase is a major barrier to transformation of V. cholerae. Transformation of wild type and DNase-negative strains of V. cholerae was accomplished by electroporation. Efficiency of transformation by electroporation increased with field strength, decreased with plasmid size, and was relatively insensitive to changes in the electrolyte composition of the buffer as long as isotonic sucrose was present. Host-controlled modification/restriction systems also affected transformation efficiency in V. cholerae. 相似文献
19.
David O'Callaghan Duncan Maskell Julian E. Beesley M.Robert Lifely Ian Roberts Graham Boulnois Gordon Dougan 《FEMS microbiology letters》1988,52(3):269-274
Abstract Plasmid pKT274 encoding a determinant for the Escherichia coli K1 polysaccharide was introduced into the Salmonella typhimurium aro A vaccine strain SL3261 and cells harbouring the plasmid were shown to express K1 polysaccharide at their cell surface. SL3261 (pKT274) could be detected in the livers and spleens of BALB/c mice infected by the intravenous route and viable organisms persisted for several weeks. SL3261 (pKT274) was cleared from the livers more rapidly and from the spleens more slowly than SL3261. Unlike mice infected with SL3261 those infected with SL3261 (pKT274) did not exhibit gross splenomegaly during the first three weeks after infection. Mice vaccinated with viable SL3261 (pKT274) were protected against challenge with virulent S. typhimurium but failed to produce detectable levels of humoral anti-K1 polysaccharide antibodies. 相似文献
20.
Julian J. Dodson 《Environmental Biology of Fishes》1988,23(3):161-182
Synopsis Fish migration may be viewed as the product of two processes; the selection and tracking of optimal environmental conditions
through time and space, and the use of predictive information about environmental structure to bias movements towards a goal.
The establishment and maintenance of directional bias is based on the interaction of experience and instinct. The preoccupation
of much fish orientation research with innate fixed patterns of behavior on one hand and hydrodynamics on the other has led
us to underestimate the possibility that orientation is a flexible process relying on developmental sequences, calibration
of the motor-sensory interaction based on experience and the learning of environmental pattern. Evidence illustrating how
experience and learning may influence the direction of movement and how the goal is recognized is presented according to two
general categories: (a) imprinting and early experience and (b), spatial learning, including the social transmission of migratory
routes and directions. In the first category, the olfactory hypothesis of salmon homing is briefly reviewed and new data presented
describing olfactory imprinting in Atlantic salmon,Salmo salar. In the second category, evidence is presented demonstrating the modifiability of sun-compass orientation and the ability
of some fish species to learn the spatial distribution of landmarks. The role of social transmission in the migration of coral
reef fishes is reviewed. The possible role of these learning phenomena in the formation of familiar area maps, route-based
and location-based navigation and the critical distance factor is considered. The relationship between life history and the
nature of learning in migratory orientation is discussed 相似文献